After vacuum impregnation of 13 apple cultivars:
quercetin content
ranged between 368 and 604 μg/g dry
matter. Quercetin
glycosides were introduced to apple tissue by Schulze et al. (2012)
Increase by 0.36 log for Saccharomycescerevisiae, 0.73
log for Lactobacillus acidophilus and 1.07 log for Phomaglomerata for
vacuum impregnated sample in comparison to soaking sample. The authors applied
vacuum fortification of tissue at different pressure levels, in each case
obtaining the level of bacteria over 107 CFU/g. Rodriguez (1998) applied vacuum
impregnation with isotonic sugar solutions supplemented with Saccharomycescerevisiae,
Lactobacillus acidophilus and Phomaglomerata.
over 106 CFU/g Lactobacillus casei in air
dried (40 °C) product. Betoret et al.
(2003) applied vacuum impregnation of apples with apple juice supplemented with Saccharomycescerevisiae or milk
inoculated with Saccharomycescerevisiae and
Lactobacillus casei
After vacuum impregnation:109 CFU/gof tissue. The
matrix consisted of cylindrical pieces of Granny Smith apples. The author
applied one vacuum pulse at five levels of pressure and soaking in thesame
solutions with no vacuum application. In the case of vacuum impregnation, the
author observed a microbial growth rate higher by 0.36 log for S. cerevisiae, 0.73
log for L. acidophilus and 1.07 in the case of P. glomerata in
comparison to soaked samples. A significant effect of the content of extract in
the impregnating solution on the level of microorganisms introduced to the
tissue was found in most of the presented studies. The best results were
obtained during the application of isotonic or almost isotonic solutions. The
use of hypo- and hypertonic solutions resulted in significantly lower levels of
microorganisms in the final product. Most of the conducted studies present
vacuum impregnation as an effective tool in the production of probiotic food
based on the matrix of fruit and vegetable materials, as an alternative to
dairy products. At the same time, a significant aspect is also associated with
the method of fruit and vegetable tissue preservation after the introduction of
microorganisms, facilitating the maintenance of their high levels in the final
product. The most frequently used method in this respect, with potentially the
greatest applicability, is drying, particularly air drying and freeze-drying. Noorbakhsh et al.
(2013) introduced bacteria Lactobacillus rhamnosus to the
tissue of apple slices together with apple juice diluted withwater at
a 1:1 ratio (pH 5–5.2).
After vacuum impregnation: fruits
enriched with Ca2+ and Fe2+ ions respectively. Fito et al.
(2001) applied vacuum impregnation to introduce calcium and iron ions to the
tissue of sliced eggplant and orange rind, similarly as it was in a study by
Barrera et al. (2004) for apple tissue. Introduction of Ca2+ and Fe2+ to apple
tissue was connected with osmotic dehydration, however, low concentrations of
introduced ions did not change the kinetics of the process and the
effectiveness of calcium and iron introduction by vacuum impregnation was
comparable in hypo- and hypertonic solutions.
An increase in calcium content from 0%
to 40% of the recommended daily intake for an adult per 200g of apples. Barrera et al. (2009) applied osmotic
dehydration to calcium enriched slices of apple cv. Granny Smith.
Greater
incorporation at pressures of 14 and 17 kPa, levels
of microorganisms over 107 CFU/g. Maguina et al.
(2002) impregnated apple slices with a sugar solution containing
approximately108 CFU/g of Bifidobacterium ssp.