Smaller ice
crystals in AFP-I impregnated
(58 kPa, for 5
min) frozen samples. The
authors found a reduction of carboxyl groups esterified with
methyl alcohol in the tested tissue in the
range from 85% to 45% in comparison to water-impregnated apples.
The relevant literature data suggest that
in the case of freezing an alternative to calcium ions and pectin
methyl esterase may be provided by the application of antifreeze protein type I
in the impregnating solutions. Antifreeze
proteins I were used in the impregnation of
watercress e.g., by Cruz et al. (2009).
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